Microbiology (BIO 108) –  Chapter 3: Microscopy

 

 

Learning Objectives

 

1.   Define the metric units used for the measurement of microorganisms and know their

      U.S. equivalents.

 

2.   Define total magnification and image resolution.

 

3.   Diagram the path of light through a compound microscope.

 

4.   Identify a use for darkfield, phase-contrast, differential interference, and fluorescence

      microscopy, and compare each with brightfield microscopy.

 

5.   Explain how electron microscopy differs from light microscopy.

 

6.   Distinguish between transmission electron microscopy and scanning electron

      microscopy.

 

7.   Distinguish between an acidic dye and a basic dye.

 

8.   Compare simple, differential and special stains and give two examples of each.

 

9.   List the steps of the Gram stain, and describe the appearance of gram-positive and

      gram-negative cells after each step.

 

10. Which diseases can the Gram stain be used to detect?  

 

11. Compare and contrast the Gram stain and acid-fast stain.

 

12. Describe uses for each of the following: capsule stain, endospore stain, flagella

      stain.

 

13. Define all the boldface terms in chapter three and add them to your vocabulary.